Serum low replacement medium versus serum rich replacement medium for production of Anti-Schistosoma Monoclonal antibody: A comparative study
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چکیده
منابع مشابه
Enhancement in colonization of bovine spermatogonial stem cells following addition of knock-out serum replacement to culture medium
Enrichment of cell suspension with germ cells prior to injection into recipient seminiferous tubules is of importance in spermatogonial stem cells (SSCs) transplantation. Knock-out serum replacement (KSR) has been reported to enhance the proliferation of murine SSCs and human embryonic stem cells. The aim of the present study was to investigate the effect of KSR versus fetal bovine serum (FBS) ...
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For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency ...
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A calf serum ultrafiltrate fraction permitted growth for at least 3.5 generations, including one subculture, of MRC-5 cells in defined medium in the absence of whole serum. The active material has a molecular weight of 10 000 Daltons or less. This suggests that there may be no requirement for a large macromolecular component of serum. The ultrafiltrate was assayed by maximum cell yield from a s...
متن کاملPlatelet Rich in Growth Factors (PRGF): A Suitable Replacement for Fetal Bovine Serum (FBS) in Mesenchymal Stem Cell Culture
Background: Due to high differentiation potential and self-renewality, Mesenchymal Stem Cells are now widely considered by researchers in several diseases. FBS is used as a supplement in culture media for proliferation, differentiation, and other culture processes of MSCs, which is associated with transmission risk of a variety of infections as well as immune responses. PRGF derived from platel...
متن کاملUse of chicken serum as a good replacement for the fetal calf serum in cultivation of promastigotes of Leishmania major
Genus Leishmania is a protozoan parasite that causes different severe diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, for mass cultivation and the most expensive ingredient in these media. In the present work, the efficacy of chicken serum was evaluated in Leishmania culture media. The results indicated that, the (10%) chicken serum enriched culture medium su...
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ژورنال
عنوان ژورنال: Clinical Epidemiology and Global Health
سال: 2020
ISSN: 2213-3984
DOI: 10.1016/j.cegh.2019.09.014